Dietary supplementation with Bacillus-based probiotic improves gut health in the weaned piglets challenged by rotavirus

作者：Fengyu Xiang, Heng Yang, Xiangqi Fan, Dayan Tan, Bing Huang, Bing Yu, Jun He, Yuheng Luo, Junqiu Luo, Hui Yan, Junning Pu, Jianping Wang, Quyuan Wang, Huifen Wang, John K Htoo, S Maria Mendoza, Guiling Yan, Xiangbing Mao · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2025 · DOI：10.1186/s40104-025-01286-7 · 被引用次数：2 · 研究领域：Viral gastroenteritis research and epidemiology、Animal Nutrition and Physiology、Probiotics and Fermented Foods

Abstract Background As probiotics, Bacillus strains may regulate some physiological functions in animals. This study aimed to evaluate whether dietary supplementation with a Bacillus -based probiotic could alleviate gut damage induced by rotavirus (RV) infection in piglets. Twenty-four piglets were randomly assigned into 2 groups fed with the basal diet ( n = 16) and the diet containing 10 9 colony-forming unit Bacillus spores/kg ( n = 8). On d 8, 8 piglets fed with the diet supplemented with Bacillus -based probiotic and 8 piglets fed with basal diet were orally infused with RV, while the residue piglets had oral gavage of sterile essential medium. The trial duration was 12 d. Results RV challenge induced diarrhea, significantly destroyed the morphology of jejunal mucosa ( P < 0.05), significantly increased RV-antibody and RV non-structural protein 4 of jejunal mucosa ( P < 0.05), significantly impaired antioxidant capacity (including malondialdehyde level, total antioxidant capacity and catalase activity), immunity (such as interleukin 2, interleukin 4 and secreted immunoglobulin A levels), mucins and the mRNA expression of tight-junction-related (such as Zonula occludens 1, occludin) and apoptotic-related (including B-cell lymphoma/leukaemia-2-associated X protein, B cell lymphoma/leukaemia-2, cysteinyl aspartate specific proteinases) genes of jejunal mucosa ( P < 0.05), and, to some extents, affected the bacteria community structure and abundance of ileal digesta...