Bambara Groundnut Pericarp–Derived Carbon Dots: Reinforcement of Gelatin/PLA Bilayer Films and Their Function as Preservative in Packed Asian Seabass Slices

作者：G. Murugan, Krisana Nilsuwan, Jun Tae Kim, Jong‐Whan Rhim, Tao Yin, Bin Zhang, Soottawat Benjakul · 发表于：Journal of Food Science · 年份：2025 · DOI：10.1111/1750-3841.70469 · 被引用次数：12 · 研究领域：Microplastics and Plastic Pollution、biodegradable polymer synthesis and properties

Conversion of agro-waste into carbon dots (CDs) transforms biomass into sustainable nanomaterials, enabling advanced applications in packaging and ensuring food safety. Bambara groundnut pericarp powder (BGPP) was hydrothermally processed at 200°C for varying durations (3-12 h) to synthesize BGP-CDs. BGP-CDs exhibited differences in size (1.34-15.02 nm), shape, and chemical composition, as characterized by transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) analyses. All samples exhibited excellent optical properties, including photoluminescence and UV barrier capability. Notably, BGP-CDs/3 (synthesized for 3 h) at 100 µg/mL demonstrated the highest UV-blocking efficiency against UV-A (35.62%) and UV-B (92.64%) lights. BGP-CDs synthesized for 12 h exhibited remarkable antimicrobial activities against selected species (Escherichia coli, Listeria monocytogenes, Aspergillus flavus, and Aspergillus parasiticus) due to their nanoscale size; however, their reduced size negatively impacted human cell viability. Antioxidant activity was highest in BGP-CDs synthesized for 6 h (BGP-CDs/6), as determined by different assays. Incorporating BGP-CDs/6 (4%, w/w) into gelatin/polylactic acid (PLA) bilayer film enhanced UV and water barrier properties, antioxidant capacity, and antibacterial efficacy compared to the control film. Additionally, Asian seabass slices (AS-S) packaged in pouches made from active bilayer film had the maintained color with negligible pH chan...