Pretreatment of sweet sorghum silages with Lactobacillus plantarum and cellulase with two different raw material characteristics: fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities

作者：Zhiqiang Sun, Yuan Li, S.L. Li, Siyuan Wang, Siyi Li, Ke Yan, Run Gao, Lei Wang, Zhenming Zhou, Zhe Wu, Zhu Yu · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2025 · DOI：10.1186/s40538-025-00749-3 · 被引用次数：10 · 研究领域：Ruminant Nutrition and Digestive Physiology、Agroforestry and silvopastoral systems、Bioenergy crop production and management

Sweet sorghum (Sorghum bicolor (L.) Moench) is a popular forage crop in arid and semi-arid areas due to its high drought tolerance, rapid growth, and low production costs. In addition, sweet sorghum, has relatively specific ensiling characteristics because of its high moisture content and sufficient amount of water soluble carbohydrates. Therefore, it is crucial to investigate the raw material characteristics and exogenous additive pretreatment for the regulation of silage quality. This study aimed to evaluate the effects of Lactobacillus plantarum (LP) and cellulase (CEL) on the fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities of sweet sorghum silages with two different raw material characteristics (fresh sweet sorghum material (MC1); sweet sorghum material wilted outdoors for 6 h after chopping (MC2)). In this study, the sweet sorghum treatments were: control (without additives), LP, CEL, or a combination of LP and CEL (LP_CEL). All treated sweet sorghum samples were ensiled for 30 d. A higher content of lactic acid, propionic acid, D-fructose, glucose, sucrose and a lower content of structural carbohydrates were observed in MC1 silage than in MC2 silage. In MC2 silage, the addition of CEL or LP_CEL decreased the content of structural carbohydrates, while it increased the content of D-fructose, glucose, D-arabinose, xylitol, maltose and trehalose (P < 0.05). The in vitro gas production at 48 h was greater in MC1 silage t...