Locking-Fluorescence Signals Regulated CRISPR/Cas12a Biosensor Based on Metal–Organic Framework for Sensitive Detection of Salmonella typhimurium

作者：Yan Zhao, Qianqian Yu, Miaolin Duan, Qi Zhang, Zhishang Li, Yan Zhang, Yana Liu, Han Wang, Xingmin Li, Ruitong Dai, Fei Jia · 发表于：Journal of Agricultural and Food Chemistry · 年份：2024 · DOI：10.1021/acs.jafc.4c07681 · 被引用次数：18 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、CRISPR and Genetic Engineering

The efficient, sensitive, and rapid detection of Salmonella typhimurium ( S. typhimurium ) in food and food products is important to ensure food safety and health. This study developed a fluorescence biosensing assay that integrated recombinase-aided amplification (RAA) and CRISPR/Cas12a with a zeolitic imidazolate framework-8@fluorescein sodium (ZIF-8@FLS) nanocomposite for the sensitive detection of S. typhimurium . In this approach, using RAA as a preamplification module, CRISPR/Cas12a-AChE as a target recognition and dual-enzyme cascade amplification module, and the prepared ZIF-8@FLS with high porosity and rapid pH responsiveness as a fluorescence signal explosive amplification module, the RAA-CRISPR/Cas12a-ZIF-8@FLS biosensor was constructed. Under optimal conditions, it exhibited an excellent linear relationship for S. Typhimurium, with a sensitive detection limit as low as 1.3 × 10 2 CFU/mL and could complete sample detection within 2 h relying on the RAA and ZIF-8@FLS explosive fluorescence rapid response, demonstrating its significant advantages in specificity, sensitivity, and reliability in food-borne pathogens detection.