Investigation of Anti-Inflammatory Properties, Phytochemical Constituents, Antioxidant, and Antimicrobial Potentials of the Whole Plant Ethanolic Extract of Achillea santolinoides subsp. wilhelmsii (K. Koch) Greuter of Balochistan

作者：Ali Akbar, Zareen Gul, Su Hlaing Chein, Muhammad Bilal Sadiq · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2023 · DOI：10.1155/2023/2567333 · 被引用次数：16 · 研究领域：Essential Oils and Antimicrobial Activity、Phytochemistry and Biological Activities、Phytochemicals and Antioxidant Activities

Medicinal plants are rich source of phytochemical constitutes and can be used to treat many human diseases. Infectious diseases have always been a major source of concern. Globally, the medicinal plant Achillea wilhelmsii locally known as Bohe Madran is extensively dispersed and widely used as traditional medicine. The aim of this present work is to investigate phytochemical constituents and antimicrobial, antioxidant, and anti-inflammatory properties of the whole plant ethanolic extract of Achillea santolinoides subsp. wilhelmsii (WEEAW) from Balochistan region. The total phenolic content was 14.81 ± 0.18 mg GAE/g of the extract whereas the total flavonoid content was 12.27 ± 0.12 mg QE/g of the extract. The antioxidant ability of the extract was analyzed by DPPH (2,2-diphenyl-1-picryl-hydrazyl) scavenging assay and FRAP (ferric reducing antioxidant power) assay in terms of concentration having 50% inhibition (IC50). Results showed that IC50 value for DPPH% inhibition was 0.367 ± 0.82 mg/mL while FRAP assay represented with IC50 value of 0.485 ± 1.26 mg/mL. In antileishmanial bioassay, the extract was analyzed against Leishmania major and showed good activity with IC50 value of 7.02 ± 0.83 mg/mL. Antibacterial assay revealed that Staphylococcus aureus was highly sensitive with the diameter of inhibition zone ( 21.61 ± 1.09 mm) followed by Salmonella typhi ( 17.32 ± 0.15 mm), Pseudomonas aeruginosa ( 16.41 ± 0.63 mm), and Escher...