Bio‐Functional Potential and Biochemical Properties of Propolis Collected from Different Regions of Balochistan Province of Pakistan

作者：Ali Akbar, Zareen Gul, Saliha Aziz, Muhammad Bilal Sadiq, Jahangir Khan Achakzai, Shazia Saeed, Su Hlaing Chein, Hassan Sher · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2022 · DOI：10.1155/2022/7585406 · 被引用次数：14 · 研究领域：Bee Products Chemical Analysis、Essential Oils and Antimicrobial Activity、Phytochemicals and Antioxidant Activities

Propolis is a well‐known resinous natural substance collected by honeybees ( Apis mellifera L.) from plants exudations. Variations in chemical composition of propolis are due to different sources from which it is collected and change in climate and geographical location. In this study, different propolis samples were collected from different regions of Balochistan and examined for its chemical composition, total phenolics and total flavonoid contents, and antioxidant potential by using DPPH radical scavenging assay and antimicrobial activity. Bioactive components analysis revealed the presence of steroids, carbohydrates, flavonoids, coumarins, cardiac glycosides, quinones, anthraquinones, terpenoids, tannins, and phlobatannins at different levels. The total phenolics contents were ranged from 2.9343 ± 1.247 to 6.0216 ± 2.873 mg GAE g -1 , and flavonoid contents were found to be 0.1546 ± 0.087 to 0.6586 ± 0.329 mg QE g -1, respectively. The antioxidant ability of each extract was analyzed by their concentration having 50% inhibition ( I C 50 ). The propolis sample P3 possessed lower I C 50 27.07 ± 0.73 mg mL −1 with higher % inhibition of DPPH radical, and P8 showed lower % inhibition by having I C 50 84.43 ± 2.07 mg mL −1 . The antibacterial activity of all samples was analyzed against a wide group of bacteria including Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , and Klebsiella pneumonia and propolis extract (P4) was highly active against Klebsiella pn...