The polyphenol-rich extract from chokeberry (Aronia melanocarpa L.) modulates gut microbiota and improves lipid metabolism in diet-induced obese rats

作者：Zhu Feng Yue, Jiaying Zhang, Yulong Wei, Jingyi Hao, Yuqing Lei, Wanbin Zhao, Yuhang Xiao, Aidong Sun · 发表于：Nutrition & Metabolism · 年份：2020 · DOI：10.1186/s12986-020-00473-9 · 被引用次数：76 · 研究领域：Gut microbiota and health、Diet and metabolism studies、Liver Disease Diagnosis and Treatment

Abstract The gut microbiota plays a critical role in obesity and lipid metabolism disorder. Chokeberry ( Aronia melanocarpa L . ) are rich in polyphenols with various physiological and pharmacological activities. We determined serum physiological parameters and fecal microbial components by using related kits, liquid chromatography-mass spectrometry (LC-MS) and 16S rRNA gene sequencing every 10 days. Real-time PCR analysis was used to measure gene expression of bile acids (BAs) and lipid metabolism in liver and adipose tissues. We analyzed the effects of different Chokeberry polyphenol (CBPs) treatment time on obesity and lipid metabolism in high fat diet (HFD)-fed rats. The results indicated that CBPs treatment prevents obesity, liver steatosis and improves dyslipidemia in HFD-fed rats. CBPs modulated the composition of the gut microbiota with the extended treatment time, reducing the Firmicutes / Bacteroidetes ratio (F/B ratio) and increasing the relative abundance of Bacteroides , Prevotella , Akkermansia and other bacterial species associated with anti-obesity properties. We found that CBPs treatment gradually decreased the total BAs pool and particularly reduced the relative content of cholic acid (CA), deoxycholic acid (DCA) and enhanced the relative content of chenodeoxycholic acid (CDCA). These changes were positively correlated Bacteroides , Prevotella and negatively correlated with Clostridium , Eubacterium , Ruminococcaceae . In liver and white adipose tissues, the...