High-Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format

作者：Dejian Huang, Boxin Ou, Maureen Hampsch‐Woodill, Judith A. Flanagan, Ronald L. Prior · 发表于：Journal of Agricultural and Food Chemistry · 年份：2002 · DOI：10.1021/jf0201529 · 被引用次数：1573 · 研究领域：Viral Infectious Diseases and Gene Expression in Insects、Analytical Chemistry and Chromatography、Electrochemical sensors and biosensors

The oxygen radical absorbance capacity (ORAC) assay has been widely accepted as a standard tool to measure the antioxidant activity in the nutraceutical, pharmaceutical, and food industries. However, the ORAC assay has been criticized for a lack of accessibility due to the unavailability of the COBAS FARA II analyzer, an instrument discontinued by the manufacturer. In addition, the manual sample preparation is time-consuming and labor-intensive. The objective of this study was to develop a high-throughput instrument platform that can fully automate the ORAC assay procedure. The new instrument platform consists of a robotic eight-channel liquid handling system and a microplate fluorescence reader. By using the high-throughput platform, the efficiency of the assay is improved with at least a 10-fold increase in sample throughput over the current procedure. The mean of intra- and interday CVs was