Advances in In Situ Probing of Plant Cell Wall Polysaccharides

作者：Ningyu Lei, Xin Wang, Shikang Chen, Xinru Liu, Xingtao Zhou, Yujun Wan, Xiaoxiao Song, Weiwei He, Shao-Ping Nie, Jun-Yi Yin · 发表于：Journal of Agricultural and Food Chemistry · 年份：2026 · DOI：10.1021/acs.jafc.5c10389 · 被引用次数：1 · 研究领域：Polysaccharides and Plant Cell Walls、Plant Surface Properties and Treatments、Advanced Cellulose Research Studies

The plant cell wall forms the structural framework and load-bearing component, which is crucial for the growth and differentiation of plant tissues. It is composed of dynamic, interconnected networks of polysaccharides, glycoproteins, and lignin. Polysaccharides play a critical role in regulating cell wall assembly, mechanics, and growth. Accurate and rapid visualization of polysaccharide distribution within plant cell walls is increasingly important for refining structural models and advancing bioenergy industrialization. This review highlights widely applied techniques for in situ characterization of cell wall polysaccharides at the tissue or single-cell level, including immunofluorescence labeling, Raman imaging, infrared spectroscopic imaging, atomic force microscopy, mass spectrometry imaging, and solid-state nuclear magnetic resonance, accompanied by examples to illustrate their practical utility. Special emphasis is placed on the underlying principles and applications in elucidating the architecture and biological functionality of polysaccharides within plant cell walls, thereby underscoring their relevance in advancing both fundamental and applied plant science research.