Process optimization and antioxidant activity of white peony root components extracted via ultrasound-assisted deep eutectic solvents

作者：Xin Li, Ammara Sohail, Zihan Li, Yan Cheng, Yilu Wang, Li Cui, Hidayat Hussain, Zheng Wang, Wenshuang Zhao, Jinhua Du, Yue Li, Jixiang He, Daijie Wang · 发表于：Ultrasonics Sonochemistry · 年份：2025 · DOI：10.1016/j.ultsonch.2025.107685 · 被引用次数：3 · 研究领域：Ionic liquids properties and applications、Phytochemicals and Antioxidant Activities、Heavy Metals in Plants

The study aimed to extract antioxidant-rich components from white peony root (WPR) using the ultrasound-assisted deep eutectic solvents (UAE-DES) and optimizing them through response surface methodology to enhance their antioxidant potential. Twenty DESs and three conventional solvents were evaluated, and choline bromide-formic acid (DES17) was found best among them for extracting antioxidant components from WPR. The UAE-DES process was optimized using the Box-Behnken response surface method, achieving a maximum yield (18.16 ± 0.67 mg/g) under optimal conditions (41 min, 1:40 g/mL, and 58 % DES/H 2 O), with an extraction efficiency 4–13 times higher than traditional techniques. The study also evaluated the significant DES reusability and recovery of antioxidant components using macroporous resins. Additionally, the DES-derived optimized WPR extract demonstrated significant in vitro antioxidant activity. FTIR, 1 H NMR, SEM, and DFT calculations were carried out to better understand chemical component interactions and extraction mechanisms. This study advances green solvent chemistry and ultrasound applications in natural product processing by providing a viable, environmentally friendly method for the quick and effective extraction of important phytochemicals from plant matrices