Multifunctional signal probe-based immunochromatographic test strip for rapid detection of aflatoxin B1 in complex matrix samples

作者：Wenjie Ren, Ting Wang, Jiarui Pang, Yifan Li, Yuxiu Han, Baoshan He, Renyong Zhao, Min Wei, Wenhong Zhao, Zhiguang Suo, Yiwei Xu, Zhui Tu · 发表于：Food Chemistry X · 年份：2025 · DOI：10.1016/j.fochx.2025.103255 · 被引用次数：6 · 研究领域：Biosensors and Analytical Detection、Mycotoxins in Agriculture and Food、Advanced Biosensing Techniques and Applications

Immunochromatographic test strips (ICTS) are widely used for the immediate and rapid detection of toxins; however, they are prone to false-positive results and insufficient sensitivity when applied to complex sample matrices. To overcome these limitations, herein, a multifunctional signal probe-based ICTS was developed for the rapid detection of aflatoxin B 1 (AFB 1 ) in complex matrix samples by conjugating biotinylated nanobodies (G8-Bio) with streptavidin-modified magnetic beads (MB-SA) as the probes. The constructed ICTS achieved a limit of detection (LOD) of 0.063 ng/mL for AFB 1 . In corn spiked experiments, the results ranged from 98 % to 102 %, with coefficients of variation (CV) between 1.4 % and 6.86 %, confirming the reliability of this method. Furthermore, the IC 10 value in soy sauce samples was 0.069 ng/mL, indicating its suitability for detecting AFB 1 in dark-colored matrices. This approach provides a novel strategy for the rapid and accurate detection of trace toxins in complex matrix samples. • A nanobody-based test strip with anti-interference properties was developed for aflatoxin B 1 (AFB 1 ) detection. • The designed multifunctional signal probe simultaneously facilitates AFB 1 enrichment, specific recognition, and signal amplification. • The signal probes effectively mitigate background interference caused by sample impurities on the detection strip. • This test strip demonstrates a satisfactory detection limit and high specificity.