Antimicrobial activity and possible mechanisms of juglone against Escherichia coli, Staphylococcus aureus, and Salmonella pullorum

作者：Lei Wang, Mingxin Qiu, Xuanyue Li, Mengjian Liu, Luyu Li, Yong Chen · 发表于：BMC Microbiology · 年份：2025 · DOI：10.1186/s12866-025-04354-0 · 被引用次数：4 · 研究领域：Bioactive Compounds and Antitumor Agents、Synthesis and bioactivity of alkaloids、Beetle Biology and Toxicology Studies

BACKGROUND: Escherichia coli, Staphylococcus aureus, and Salmonella pullorum are significant pathogens that threaten livestock and poultry health. Although antibiotics and synthetic antimicrobial agents can combat these pathogens, antibiotic resistance remains a major concern. Recent decades have seen growing interest in antibiotic alternatives. Juglone, a natural naphthoquinone compound from Juglandaceae plant, exhibits strong antimicrobial activity against S. aureus. However, its antimicrobial mechanism is not yet fully understood. This study investigated the antimicrobial mechanism of juglone from the perspectives of cell biology, cell morphology, and transcriptomics. RESULTS: Juglone had potent antimicrobial effects against E. coli, S. aureus, and S. pullorum. The minimum inhibitory concentration (MIC) of juglone against all three bacterial strains was 15.6 µg/mL. Treatment with juglone decreased bacterial metabolic activity, reduced the intracellular DNA and RNA fluorescence intensity, resulted in the leakage of intracellular alkaline phosphatase (AKP) and ions, and caused a decline in ATP content and ATPase activity. Scanning electron microscopy (SEM) revealed significant membrane damage in each of the three bacterial species following juglone treatment. Transcriptomic sequencing and Gene Ontology (GO) enrichment analysis of S. pullorum revealed that juglone treatment resulted in a significant upregulation of GO terms related to translation, while those terms associated...