Characterization of Biocalcium Microparticles from Saltwater Crocodile (Crocodylus porosus) Bone and Their Potential for Enhancing Fish Bologna Quality

作者：Theeraphol Senphan, Natthapong Mungmueang, Supatra Karnjanapratum, Sutee Wangtueai, Akkasit Jongjareonrak, Suthasinee Yarnpakdee · 发表于：Foods · 年份：2025 · DOI：10.3390/foods14101732 · 被引用次数：3 · 研究领域：Aquaculture Nutrition and Growth、Meat and Animal Product Quality、Ichthyology and Marine Biology

Saltwater crocodile (SC; Crocodylus porosus) bone, an underutilized by-product, can be converted into high-value bio-calcium (Biocal), serving as a potential source of calcium and minerals. This study aimed to produce SC bone Biocal as functional gel enhancer for fish bologna development and to increase calcium intake. The resulting bone powder was evaluated for physicochemical, microbiological, and molecular properties. Additionally, the textural, physicochemical, structural, and sensorial properties of the formulated fish bologna incorporating Biocal at varying levels (0–10% w/w) were also evaluated. Biocal, obtained as a fine white powder, had a 16.83% yield. Mineral analysis showed 26.25% calcium and 13.72% phosphorus, with no harmful metals or pathogens detected. X-ray diffraction confirmed hydroxyapatite with 69.92% crystallinity, while calcium bioavailability was measured at 22.30%. Amino acid analysis indicated high levels of glycine, proline, and hydroxyproline, essential for collagen support. The findings confirmed that SC bone Biocal is beneficial and safe for food fortification. Incorporating SC Biocal (2–10% w/w) significantly affected the fish bologna characteristics (p < 0.05). As the Biocal level increased, the gel strength, hardness, and shear force also increased. The addition of 6% (w/w) Biocal significantly improved the textural property, without a detrimental effect on the sensory attributes of the bologna gel (p < 0.05). SDS-PAGE analysis showed TG...