Structural Optimization of Pyrazole Compounds as Hsp90 Regulators with Enhanced Antitumor Activity

作者：Ziwen Feng, Li Li, Shi-Duo Zhang, Wang Ying-ji, Jia-Yue Pei, Nannan Chen, Bei-Duo Wu, Qiuling Zheng, Qidong You, Xiaoke Guo, Xiaoli Xu · 发表于：Journal of Medicinal Chemistry · 年份：2025 · DOI：10.1021/acs.jmedchem.4c02182 · 被引用次数：4 · 研究领域：Heat shock proteins research、Computational Drug Discovery Methods、ATP Synthase and ATPases Research

Targeting Hsp90 is an effective strategy for cancer therapy. TAS-116 has been approved for the treatment of gastrointestinal stromal tumors. Our previous studies identified a series of pyrazole derivatives as covalent Hsp90 inhibitors that allosterically disrupt the Hsp90-Cdc37 interaction. Here, through systematic structure–activity relationship (SAR) optimization, compound 39 ( DDO-6691 ) with a new covalent warhead was developed, which demonstrates improved ADME properties and significantly enhanced antitumor activity. Notably, parental HCT-116 cells exhibited markedly greater sensitivity to compound 39 (IC 50 > 50 μM) compared to their Cdc37-knockout counterparts. Importantly, compound 39 displayed potent tumor growth inhibition in HCT-116 xenograft mouse models. These collective findings underscore the therapeutic promise of covalent Hsp90-targeted disruption of the Hsp90-Cdc37 complex, offering a novel mechanistic approach to cancer treatment.