M2 macrophage-derived exosome-modified bioprosthetic heart valves with enhanced anti-inflammatory, anticoagulant and anticalcification properties

作者：Jingruo Chen, Shufen Li, Xiaoting Chen, Tianyi Qu, Ming Chen, Ming Chen, Zheng Chai, Weihua Zhuang, Walter Hu, Mao Chen, Mao Chen · 发表于：Materials & Design · 年份：2025 · DOI：10.1016/j.matdes.2025.114018 · 被引用次数：4 · 研究领域：Cardiac Valve Diseases and Treatments、Electrospun Nanofibers in Biomedical Applications、Infectious Aortic and Vascular Conditions

Exosomes derived from M2 macrophages (M2-EXO) were used to modify glutaraldehyde cross-linked bovine pericardium (Glut-BP), which endowed Glut-BP with outstanding biocompatibility, anti-inflammatory, anti-thrombotic and anti-calcification properties. • Exosomes derived from M2 macrophages were introduced to prepared a novel bioprosthetic heart valves (M2-EXO-BP). • M2-EXO-BP presented good biocompatibility and effectively decreases inflammatory. • M2-EXO-BP demonstrated reduced blood platelet adhesion and good anti-thrombotic performance. • M2-EXO-BP obviously reduced calcification in subcutaneous implantation experiment. Glutaraldehyde (Glut) cross-linked bioprosthetic heart valves (BHVs) have shown great performance in the replacement treatment of severe valvular heart diseases in recent years due to the rapid development of transcatheter aortic valve replacement (TAVR) and the aging population. However, the durability of BHVs suffers from inflammation, limited endothelialization, thrombus formation and calcification during clinical application, which makes it urgently to prolong the service life of BHVs. To this end, we introduced exosomes derived from M2 macrophages to the surface of Glut cross-linked bovine pericardium (Glut-BP) to develop a novel kind of BHVs, namely M2-EXO-BP, to improve the durability of Glut-BP. Compared with Glut-BP, M2-EXO-BP presented significantly improved biocompatibility and endothelialization. Moreover, M2-EXO-BP also reduced the adhesion of p...