Interkingdom multi-omics analysis reveals the enhancing effects of magnesium oxide nanoparticles and Piriformospora indica co-application on tobacco growth and Ralstonia solanacearum resistance

作者：Han Li, Xiaodong He, Yu Ye, Heng Zhang, Qiang Li, Zhiyao Wang, Xi Wu, Weichang Gao, Qingcheng Qiu, Jingwei Zhu, Changying Liu, Xiang Li, Yanxia Liu · 发表于：Industrial Crops and Products · 年份：2025 · DOI：10.1016/j.indcrop.2025.121050 · 被引用次数：4 · 研究领域：Plant-Microbe Interactions and Immunity、Nematode management and characterization studies、Legume Nitrogen Fixing Symbiosis

Tobacco ( Nicotiana tabacum L.) is an important cash crop, but its yield, quality, and profitability are severely affected by bacterial wilt caused by Ralstonia solanacearum . There is currently no effective means to control tobacco bacterial wilt. This study reported a novel strategy to improve resistance to bacterial wilt and enhance the growth of tobacco. Magnesium oxide nanoparticles with concentrations of > 250 mg/L inhibited the growth of Ralstonia solanacearum while promoting Piriformospora indica growth. Magnesium oxide nanoparticles can bind to Piriformospora indica through electric field force. Compared with the single application of magnesium oxide nanoparticles or Piriformospora indica , co-application of magnesium oxide nanoparticles (250 mg/L) and Piriformospora indica (10 7 cfu/mL) demonstrated a superior promoting effect on tobacco growth and resistance to bacterial wilt. The magnesium oxide nanoparticles– Piriformospora indica combination strengthened the functions of magnesium oxide nanoparticles or Piriformospora indica on tobacco by inducing expression of the genes involved in pathogenesis (such as PTI and PR10a ), immunity, reactive oxygen species detoxification, hormone signaling, and transcriptional regulation (such as WRKY ). This combination shapes the microbial community to improve tobacco resistance to Ralstonia solanacearum , resulting in an increase in the abundance of beneficial bacteria such as Streptomyces , Nocardioides , and Micromonospora . ...