Lactiplantibacillus plantarum FEED8 Biosynthesis of Pyranoanthocyanin (Cyanidin-3-glucoside-4-vinylcatechol) Improves Oxidative Stress and Inflammation of the Gut Microbiome in Cadmium-Exposed Mice

作者：Qing Zhang, Yue Li, Yan Han, Weijie Zhou, Xusheng Li, Jianxia Sun, Weibin Bai · 发表于：Journal of Agricultural and Food Chemistry · 年份：2025 · DOI：10.1021/acs.jafc.5c01093 · 被引用次数：5 · 研究领域：Gut microbiota and health、Natural Antidiabetic Agents Studies、Diet and metabolism studies

The study is to explore the biosynthesis of cyanidin-3-glucoside-4-vinylcatechol (C3G_VC) through Lactiplantibacillus plantarum -fermented caffeic acid and cyanidin-3-glucoside (C3G) extract (molar ratio = 1:30) in the model medium. C3G_VC was isolated and purified by a venusil ASB-C18 column with a medium-pressure liquid chromatography (MPLC) system. The chemical structure of C3G_VC was identified by high-performance liquid chromatography (HPLC), which showed the maximum absorption wavelength of 505.57 nm. This study showed that Cd exposure of mice induced liver damage, oxidative stress, and inflammation of the gut microbiome. Our findings demonstrated that C3G_VC intervention in Cd-exposed mice significantly mitigated oxidative stress injury by declining the malondialdehyde (MDA) level and increasing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in the liver, meanwhile alleviating liver injury by decreasing the bile acid (BA) level and accelerating the excretion of fecal BA. Moreover, the Cd_C3G_VC group showed elevated levels of mRNA expression of pro-inflammatory cytokines (IL6, IL1β, and TNF-α) and inhibited BA synthesis (CYP7A1) in Cd-exposed mice. The fermentation results in vitro showed that C3G_VC had a higher residue than that of cyanidin-3-glucoside. The 16S rRNA high-throughput sequencing disclosed that C3G_VC intervention in Cd-exposed mice significantly increased the abundance of Faecalibaculum and unidentified_Lachnospiraceae . ...