The effect of supplementing Nannochloropsis oculata microalgae on ruminal fermentation, methane production and microbial population

作者：Zahra Salehian, Hamed Khalilvandi‐Behroozyar, Rasoul Pirmohammadi, Nasrollah Ahmadifard, Hadi Almasi, Germán Buendía Rodríguez, Maximilian Lackner, Abdelfattah Z. M. Salem · 发表于：Journal of Agriculture and Food Research · 年份：2025 · DOI：10.1016/j.jafr.2025.101663 · 被引用次数：4 · 研究领域：Algal biology and biofuel production、Biodiesel Production and Applications

This study aimed to evaluate the dietary supplementation of Nannochloropsis oculata (N. oculata) microalgae in different levels (1-, 2-, 3-, 4-, and 5- g/100 g dry matter (DM)) on the kinetics of gas production (GP), methane (CH 4 ) production, dry matter apparent digestibility, volatile fatty acids (VFA), and microbial population by in vitro method. The findings of this study indicated that adding N. oculata at levels of 1–5 g/100 g DM did not have an impact on GP at different incubation hours. However, a diet with 5 g/100 g DM N. oculata resulted in a consistent increase in GP rate (part c) compared to the control treatment ( P < 0.0001). Supplementing of N . oculata to the diet decreased the CH 4 production ( P < 0.001), methane to gas production ratio in 24 h ( P < 0.0001), protozoa population ( P < 0.002), total VFA ( P < 0.005), Fibrobacter succinogenes , Ruminococcus flavefaciens , Prevotella spp., Anaerovibrio lipolytica , and Butyrivibrio proteoclasticus , and increased the partitioning factor ( P < 0.0001), pH ( P < 0.001), Ruminococcus albus , Butyrivibrio fibrisolvens , B. fibrisolvens , S. ruminantium, and fungi population ( P < 0.0001). The addition of N. oculata did not impact on the levels of dry matter digestibility, ammonia nitrogen, acetate, propionate, and butyrate. However, this study demonstrated that N. oculata effectively decreased methane production and the presence of methanogenic archaea by more than 3 g/100 g DM in the diet. Therefore, N. oculata c...