Structure and metabolic function of spatiotemporal pit mud microbiome

作者：Zhihua Li, Chi Zhao, Zhenyu Mao, Fengju Zhang, Ling Dong, Chuan Song, Yao Chen, Xin Fu, Zonghua Ao, Yanfei Xiong, Hui Qin, Weizhi Song, Petri Penttinen, Suyi Zhang · 发表于：Environmental Microbiome · 年份：2025 · DOI：10.1186/s40793-025-00668-8 · 被引用次数：18 · 研究领域：Fermentation and Sensory Analysis、Gut microbiota and health、Biochemical and biochemical processes

BACKGROUND: Pit mud (PM) hosts diverse microbial communities, which serve as a medium to impart flavor and quality to Baijiu and exhibit long-term tolerance to ethanol and acids, resulting in a unique ecosystem. However, the ecology and metabolic functions of PM remain poorly understood, as many taxa in PM represent largely novel lineages. In this study, we used a combination of metagenomic analysis and chemical derivatization LC-MS analysis to provide a comprehensive overview of microbial community structure, metabolic function, phylogeny, horizontal gene transfer, and the relationship with carboxyl compounds in spatiotemporal PM samples. RESULTS: Our findings revealed three distinct stages in the spatiotemporal changes of prokaryotic communities in PM: an initial phase dominated by Lactobacillus, a transitional phase, and a final state of equilibrium. Significant variations in α- and β-diversity were observed across different spatial and temporal PM samples. We identified 178 medium- and high-quality non-redundant metagenome-assembled genomes (MAGs), and constructed their phylogenetic tree, depicting their roles in the carbon, nitrogen, and sulfur cycles. The Wood-Ljungdahl pathway and reverse TCA cycle were identified as the main carbon fixation mechanisms, with both hydrogenotrophic and aceticlastic methanogens playing a major role in methane production, and methylotrophic pathway observed in older PM. Furthermore, we identified relationships between prokaryotes and 29 ca...