PbMYB5 transcription factor plays a role in regulating anthocyanin biosynthesis in pear (Pyrus bretschneideri Rehd) skin

作者：Shangyun Li, Yutao Yang, Zhi-Wei Zhou, Xuan Zhou, Diya Lei, Ruiyuan He, Yunting Zhang, Yunting Zhang, Jiliang Zhang, Yuanxiu Lin, Yan Wang, Mengyao Li, Wen He, Qing Chen, Ya Luo, Xiaorong Wang, Haoru Tang, Yong Zhang, Yong Zhang · 发表于：Frontiers in Plant Science · 年份：2025 · DOI：10.3389/fpls.2024.1492384 · 被引用次数：5 · 研究领域：Plant Gene Expression Analysis、Plant biochemistry and biosynthesis、Phytochemicals and Antioxidant Activities

The phenylacetone pathway, which encompasses flavonoids, lignin, and other compounds, is of paramount importance in determining the quality of pear fruit. Nevertheless, the precise regulatory functions of R2R3-MYB transcription factors in the metabolic pathways that regulate pear color changes remain unclear. In this study, we isolated an R2R3-PbMYB5(PbMYB5) transcription factor from ‘Red Zaosu’ pears and demonstrated that it influenced the expression of several genes, including PbCAD1, PbF5H, PbLAR, PbANR , and PbUFGT . The overexpression of PbMYB5 resulted in a notable elevation in anthocyanin concentration within the pear epidermis. Further research has shown that PbMYB5 is able to bind to PbANS and also has interactions with PbbHLH3 and PbbHLH33 .We proposed that PbMYB5 forms a complex with PbbHLH3, PbbHLH33 , and PbWD40 to activate PbANS and promote anthocyanin accumulation. This study offers new insights into the regulation of various metabolic pathways that impact fruit coloration.