Regionalization of intestinal microbiota and metabolites in the small intestine of the Bactrian camel

作者：Yujiao Cheng, Yan Ren, Wangdong Zhang, Jia Lu, Fei Xie, Ying-Dong Fang, Xiping Fan, Wanhong He, Wenhui Wang · 发表于：Frontiers in Immunology · 年份：2024 · DOI：10.3389/fimmu.2024.1464664 · 被引用次数：7 · 研究领域：Animal Diversity and Health Studies、Gut microbiota and health、Animal health and immunology

Introduction: Peyer's patches (PPs) are crucial antigen-inductive sites of intestinal mucosal immunity. Prior research indicated that, in contrast to other ruminants, PPs in the small intestine of Bactrian camels are found in the duodenum, jejunum, and ileum and display polymorphism. Using this information, we analyzed the microbial and metabolic characteristics in various segments of the Bactrian camel's small intestine to further elucidate how the immune system varies across different regions. Methods: In this study, the microbiota and metabolite of 36 intestinal mucosal samples, including duodenal (D-PPs), jejunal (J-PPs), and ileal PPs (I-PPs), were profiled for six Bactrian camels using 16S rRNA gene sequencing and liquid chromatography with tandem mass spectrometry (LC-MS/MS). To confirm meaningful associations, we conducted connection analyses on the significantly different objects identified in each group's results. ELISA was used to analyze the levels of IgA, IgG, and IgM in the same tissues. Results: . Regarding the metabolomics, D-PPs exhibited high levels of polypeptides, acetylcholine, and histamine. On the other hand, J-PPs and I-PPs were characterized by an enrichment of free amino acids, such as L-arginine, L-glutamic acid, and L-serine. These metabolic differences mainly involve amino acid production and metabolic processes. Furthermore, the distribution of intestinal immunoglobulins highlighted the specificity of D-PPs. Our results indicated that proinflamma...