The performance of a new rapid interferon gamma release assay based on fluorescence immunochromatography for Mycobacterium tuberculosis infection testing in village doctors in China

作者：Xuefang Cao, Yanxiao Chen, Henan Xin, Jiang Du, Boxuan Feng, Yijun He, Tonglei Guo, Lingyu Shen, Yuanzhi Di, Jianguo Liang, Zihan Li, Bin Zhang, Dakuan Wang, Zisen Liu, Weitao Duan, Qi Jin, Lei Gao · 发表于：Epidemiology and Infection · 年份：2024 · DOI：10.1017/s0950268824001146 · 被引用次数：4 · 研究领域：Tuberculosis Research and Epidemiology、Mycobacterium research and diagnosis、Respiratory viral infections research

Abstract The AIMTB rapid test assay is an emerging test, which adopted a fluorescence immunochromatographic assay to measure interferon-γ (IFN-γ) production following stimulation of effector memory T cells in whole blood by mycobacterial proteins. The aim of this article was to explore the ability of AIMTB rapid test assay in detecting Mycobacterium tuberculosis (MTB) infection compared with the widely applied QuantiFERON-TB Gold Plus (QFT-Plus) test among rural doctors in China. In total, 511 participants were included in the survey. The concordance between the QFT-Plus test and the AIMTB rapid test assay was 94.47% with a Cohen’s kappa coefficient (κ) of 0.84 (95% CI, 0.79–0.90). Improved concordance between the two tests was observed in males and in participants with 26 or more years of service as rural doctors. The quantitative values of the QFT-Plus test was higher in individuals with a result of QFT-Plus-/AIMTB+ as compared to those with a result of QFT-Plus-/AIMTB- ( p < 0.001). Overall, our study found that there was an excellent consistency between the AIMTB rapid test assay and the QFT-Plus test in a Chinese population. As the AIMTB rapid test assay is fast and easy to operate, it has the potential to improve latent tuberculosis infection testing and treatment at the community level in resource-limited settings.