Combined toxicity assessment of cadmium and food-borne carbon dots on PC12 cells by metabolomics and lipidomics

作者：Zihe Qi, Haitao Wang, Mingqian Tan · 发表于：Food Science and Human Wellness · 年份：2024 · DOI：10.26599/fshw.2024.9250329 · 被引用次数：2 · 研究领域：Dye analysis and toxicity、Carbon and Quantum Dots Applications

Cadmium accumulation in seafood has become a major concern for human health. Recently, there has been an increasing focus on the potential risks associated with food-borne fluorescent carbon dots (CDs) that are formed during the thermal processing of seafood. The co-occurrence of cadmium and CDs from cooked seafood become a common phenomenon and co-exposure of them to human has been an inevitable route during long-term seafood consuming. In addition, it has been wildly recognized that CDs can be used as nanocarriers for metal ion chelation for their transport into organisms, thereby, they could influence the bioavailability of metal ion. While there have been numerous studies on the toxic effects of cadmium or CDs, none have explored the combined toxicity of food-borne CDs from clams (CCDs) and Cd 2+ . In this study, we investigated the single or co-exposure (combined exposure) of Cd 2+ and CCDs on PC12 cells to investigate the combined toxicity of them. Our analysis of cell viability revealed that CCDs significantly augmented the cytotoxicity induced by Cd 2+ . More in-depth metabolomics and lipidomics investigation indicated that the combined exposure of Cd 2+ and CCDs led to significant metabolic disorders, causing an antagonistic effect on energy metabolism, and a synergistic effect on amino acids and lipids metabolism. The disturbance in metabolomics and lipidomics was further supported by the disruption of mitochondrial membrane potential and the accumulation of react...