Bacillus velezensis B105-8, a potential and efficient biocontrol agent in control of maize stalk rot caused by Fusarium graminearum

作者：Shuang Wang, Pinjiao Jin, Yanyan Zheng, Kangkang Wang, Jiaxin Chen, Jiansheng Liu, Yonggang Li · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1462992 · 被引用次数：13 · 研究领域：Plant-Microbe Interactions and Immunity、Mycotoxins in Agriculture and Food、Plant Pathogens and Fungal Diseases

Introduction Maize stalk rot (MSR), caused by Fusarium graminearum , is the most serious soil borne disease in maize production, seriously affecting maize yield and quality worldwide. Microbial biocontrol agents are the best means of controlling MSR and reducing the use of chemical fungicides, such as Bacillus spp. Methods and results In the study, a soil-isolated strain B105-8 was identified as B. velezensis (accession No. PP325775.1 and No. PP869695.1), demonstrated a broad spectrum against various pathogens causing maize diseases, which effectively controlled MSR, exhibited a high control efficacy of more than 60% and growth-promoting effect in the pot plant. B105-8 could effectively improve soil urease (S-UE), invertase (S-SC), and catalase (S-CAT) activities. S-NP activity showed an initial increase with a peak of 20,337 nmol/h/g, followed by a decrease, but activity remained significantly better than control treatment with chemical fungicides. The application of B105-8 repaired the damage caused by F. graminearum on soil activity. The antifungal compound B-1, extracted from B105-8, was purified using a protein purifier, revealing inhibitory effects against F. graminearum . Mass spectrometry analysis indicated the potential presence of C14 Bacillomycin, C15 Iturin, C15 Mycosubtilin, C17, and C15 fengycin in B-1. In pot experiments, a 5 μL/mL concentration of B-1 exhibited 69% control on MSR, enhancing maize root elongation, elevation, and fresh weight. At 10 μL/mL, B-1 s...