Effects of xylo-oligosaccharide supplementation on the production performance, intestinal morphology, cecal short-chain fatty acid levels, and gut microbiota of laying hens

作者：Siyu Xiong, Keying Zhang, Jianping Wang, Shiping Bai, Qiufeng Zeng, Yan Liu, Huanwei Peng, Yue Xuan, Yadong Mu, Xiaobing Tang, Xuemei Ding · 发表于：Poultry Science · 年份：2024 · DOI：10.1016/j.psj.2024.104371 · 被引用次数：8 · 研究领域：Animal Nutrition and Physiology、Moringa oleifera research and applications、Rabbits: Nutrition, Reproduction, Health

This study investigated the effects of xylo-oligosaccharide supplementation on the production performance, intestinal morphology, cecal short-chain fatty acid levels, and gut microbiota of laying hens. A total of 800 Lohmann pink layers, each 48 wk old, were randomly divided into 5 dietary treatment groups, namely XOS at 0 (CON), 100 (XOS1), 200 (XOS2), 300 (XOS3) and 400 (X0S4) mg/kg. The experimental period was 24 wk. The results revealed that the egg production rate and the number of eggs laid by each layer between 1 to 12 wk increased as the XOS concentration increased ( P linear < 0.05). The sand-shell egg percentage decreased significantly from 1 to 12 wk in the XOS1, XOS2, and XOS3 groups ( P ANOVA < 0.05). Compared with the CON group, the 4 XOS dosage groups presented significant increases in the villus height and the ratio of villus height to crypt depth in the jejunum ( P ANOVA < 0.05), whereas a linear decrease in jejunal crypt depth ( P linear < 0.05) was noted. In addition, XOS supplementation significantly increased the concentrations of butyric acid and isovaleric acid in the caeca ( P ANOVA < 0.05). High-throughput sequencing analysis of bacterial 16S rRNA revealed that dietary XOS supplementation influenced the cecal microbiota. The alpha diversity analysis indicated that the richness of cecal bacteria was greater in the laying hens fed XOS. The modulation of the cecal microbiota composition upon the addition of XOS was characterized by an increased abundance...