A novel strategy for spinal cord reconstruction via vascularized allogeneic spinal cord transplantation combine spinal cord fusion

作者：Weihua Zhang, Rongyu Lan, Tingting Shen, Jie Qin, Zhihui Wang, Jiayang Chen, Jiaxing Wang, Zhuotan Wu, Yudong Xu, Yangyang Shen, Qikai Lin, Yuan Chen, Yi Wei, Yiwen Liu, Yuance Ning, Yiyan Zhou, Liji Deng, Linxuan Han, Xiaofei Wu, Haixuan Deng, Zhenbin Cao, Xianping Yao, Xiaoping Ren · 发表于：CNS Neuroscience & Therapeutics · 年份：2024 · DOI：10.1111/cns.70020 · 被引用次数：4 · 研究领域：Organ and Tissue Transplantation Research、Spinal Cord Injury Research、Veterinary Orthopedics and Neurology

AIMS: Spinal cord injuries (SCI) pose persistent challenges in clinical practice due to the secondary injury. Drawing from our experience in spinal cord fusion (SCF), we propose vascularized allogeneic spinal cord transplantation (vASCT) as a novel approach for SCI, much like organ transplantation has revolutionized organ failure treatment and vascularized composite-tissue allotransplantation has addressed limb defects. MATERIALS AND METHODS: In this study, 24 dogs were paired and underwent vASCT, with donor spinal cord grafts and polyethylene glycol (PEG) application for SCF. The experimental group (n = 8) received tacrolimus and methylprednisolone, while the control group (n = 4) received only methylprednisolone. Safety and efficacy of vASCT were evaluated through electrophysiology, imaging, and 6-month follow-up. RESULTS: The experimental group showed substantial recovery in hind limb motor function. Imaging revealed robust survival of spinal cord grafts and restoration of spinal cord continuity. In contrast, the control group maintained hind limb paralysis, with imaging confirming spinal cord graft necrosis and extensive defects. Electrophysiologically, the experimental group exhibited restored motor evoked potential signal conduction postoperatively, unlike the control group. Notably, PEG application during vASCT led to signal conduction recovery in intraoperative spinal cord evoked potential examinations for all dogs. CONCLUSION: In the vASCT surgical model, the combina...