First Report of Fusarium avenaceum Causing Root Rot of Raspberry ( Rubus corchorifolius ) in China

作者：Andéole Niyongabo Turatsinze, Xiaofan Xie, Gaofeng Chen, Ailing Ye, Liang Yue, Yun Wang, Qin Zhou, Meilan Zhang, Zongyu Zhang, Jiecai Zhao, Yubao Zhang, Ruoyu Wang · 发表于：Plant Disease · 年份：2024 · DOI：10.1094/pdis-06-24-1207-pdn · 被引用次数：7 · 研究领域：Plant Pathogens and Fungal Diseases、Plant Disease Management Techniques、Plant-Microbe Interactions and Immunity

Raspberry (Rubus corchorifolius) plants hold historical, economic, and medicinal importance in China (Yang et al. 2022). Raspberries are cultivated to generate income for local farmers in Lintao County, Dingxi City, Gansu Province. However, farmers encountered challenges due to raspberry plants exhibiting root rot disease, resulting in plant death. During a thorough field survey conducted in June 2022, symptoms ranging from leaf yellowing and wilting to necrotic lesions and root rots were observed, where approximately 30% of raspberry plants were affected. Five diseased and healthy plants were collected from the farmers' fields in Lintao (35.53oN, 103.84oE) for pathogen identification. Symptomatic and asymptomatic root tissues were surface sterilized with 75% ethanol for 30 s and 3% NaOCl for 5 min, followed by three rinses in sterile water. Small pieces (0.5 × 0.5 cm) were cut and incubated on potato dextrose agar (PDA) plates at 25°C for 7-10 days. Twenty-two pure Fusarium isolates, which displayed four distinct colony groups morphologically, were obtained. Pathogenicity tests on isolates RB10, RB1, RB30, and RB23, representing each colony group, revealed that RB10 exhibited symptoms similar to those observed in the field. The RB10 strain produced yellowish-white to greyish-white colonies on PDA and was then cultured in a carboxymethylcellulose (CMC) broth for enhanced conidia production (Zhang et al. 2020). Macroconidia were sickle-shaped or slightly curved, with three to ...