Lactobacillus cocktail and cellulase synergistically improve the fiber transformation rate in Sesbania cannabina and sweet sorghum mixed silage

作者：Tianqi Xia, Tahir Muhammad, Tianwei Wang, Yudong Wang, Xiumin Zhang, Shanji Liu, Kunling Teng, Zhihui Fu, Fangfei Yun, Siyue Wang, Sijie Jin, Jiachen Hu, Jin Zhong · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2024 · DOI：10.1186/s40538-024-00605-w · 被引用次数：20 · 研究领域：Ruminant Nutrition and Digestive Physiology、Bioenergy crop production and management、Food composition and properties

Abstract Background Elucidating the mechanism of fiber transformation underlying microbial metabolism is critical for improving fiber-rich silage digestibility and preserving silage energy for ruminant nutrient absorption. However, few studies have combined quantitative microbial function and transformation products in silage to explain this mechanism. Here, we constructed a workflow to detect the substrates and products of fiber transformation in mixed silage of Sesbania cannabina and sweet sorghum (SS) and combined the absolute quantification 16S rRNA sequencing to reveal this mechanism. Results The synergistic effect of Lactobacillus cocktail and cellulase (LC) simplified the microbial diversity and minimized the microbial quantity, making Lentilactobacillus buchneri the dominant species in SS silage . As a result, the LC-treated silage had greater lactic acid content, lower pH value, and less NH 3 -N content. The indigestible fibers were significantly decreased due to the synergistic effect of the Lactobacillus cocktail and cellulase. Changes in microbial structure during ensiling also resulted in metabolic alterations. The increased levels of microbial enzymes, including β-glucosidase and sucrose phosphorylase, involved in starch and sucrose metabolism led to the enrichment of monosaccharides (including glucose, xylose, mannose, galactose, ribose, rhamnose, and arabinose) in the LC-treated silage. We found that L. buchneri was positively associated with β-glucosidase and...