High efficient preparation of low molecular weight galactomannan from Leucaena leucocephala galactomannan through the combination of hydrogen peroxide and oxalic acid

作者：Yuheng Tao, Yuqi Zhao, Yequan Sheng, Lingyu Ruan, Wenhao Ge, Hongyan Lin, Qing Qing, Yue Zhang, Liqun Wang · 发表于：International Journal of Biological Macromolecules · 年份：2024 · DOI：10.1016/j.ijbiomac.2024.130721 · 被引用次数：7 · 研究领域：Polysaccharides Composition and Applications、Polysaccharides and Plant Cell Walls、Food composition and properties

Researchers have always been interested in polysaccharide degradation because of the increased biological activity and usability following degradation. In this work, low molecular weight galactomannan (LMW-GM) was produced through the degradation of galactomannan by H 2 O 2 and oxalic acid (OA). The optimal reaction conditions were found by conducting the response surface optimization experiment based on single-factor experiment and kinetics analysis. Under these conditions, the LMW-GM yield was 69.48 ± 1.02 %. Ultimately, an analysis of the degradation process revealed that OA attacked GM indiscriminately, and H 2 O 2 has a stronger effect on the removal of branched chains while degrading GM. Hence, the degradation steps were rearranged as H 2 O 2 was added 20 min before OA at a constant total time. The LMW-GM yield was successfully increased to 76.49 ± 1.27 %. The goal of this work is hopefully to give a theoretical foundation for the low-cost preparation and industrial production of the degradation of galactomannan.