Engineered ketoreductase-catalyzed stereoselective reduction of ethyl 2′-ketopantothenate and its analogues: chemoenzymatic synthesis of d -pantothenic acid

作者：Pan Hu, Xiaofan Wu, Yajiao Zhang, Minjie Liu, Yuan Tao, Zedu Huang, Fen‐Er Chen · 发表于：Green Chemistry · 年份：2024 · DOI：10.1039/d3gc04344d · 被引用次数：15 · 研究领域：Neurological diseases and metabolism、Enzyme Catalysis and Immobilization、Chemical Synthesis and Analysis

An asymmetric sustainable route to d -pantothenic acid was developed, centering on the reduction of ethyl 2′-ketopantothenate to ethyl ( R )-pantothenate, enabled by an E. coli strain co-expressing an engineered ketoreductase and glucose dehydrogenase.