Metabolomics analysis to interpret changes in physiological and metabolic responses to chronic heat stress in Pekin ducks

作者：Xiangyi-Yi Zeng, Arshad Javid, Gang Tian, Keying Zhang, Shiping Bai, Xue-Mei Ding, Jianping Wang, Li Lv, Yue Xuan, Shanshan Li, Qiufeng Zeng · 发表于：The Science of The Total Environment · 年份：2023 · DOI：10.1016/j.scitotenv.2023.169382 · 被引用次数：14 · 研究领域：Effects of Environmental Stressors on Livestock、Animal Nutrition and Physiology、Dietary Effects on Health

Heat stress ( HS ) is a major environmental threat that affects duck production in subtropical and tropical regions, especially in summer. This study aimed to evaluate the physiological and metabolic responses of Pekin ducks to chronic HS conditions via liquid chromatography-mass spectrometry ( LC-MS ) using a paired-fed ( PF ) experimental design. On the basis of equivalent feed intake (HS vs. PF), HS significantly reduced growth performance and the percentage of leg and breast muscles, however, markedly increased the percentage of abdominal fat and breast skin fat. Serum metabolomics results revealed that heat-stressed ducks showed enhanced glycolysis and pentose phosphate pathways, as demonstrated by higher glucose 6-phosphate and 6-phogluconic acid levels in the PF vs. HS comparison. HS decreased hepatic mRNA levels of mitochondrial fatty acid β-oxidation-related genes (MCAD and SCAD) compared to the PF group, resulting in acetylcarnitine accumulation in serum. Moreover, HS elevated the concentrations of serum amino acids and mRNA levels of ubiquitination-related genes (MuRF1 and MAFbx) in the skeletal muscle and amino acid transporter-related genes (SLC1A1 and SLC7A1) and gluconeogenesis-related genes (PCK1 and PCase) in the liver compared to the PF group. When compared to the normal control group ( NC ), HS further decreased growth performance, but it elevated the abdominal fat rate. However, increased mRNA levels of ubiquitination-related genes and serum amino acid acc...