ZnO@Ag-Functionalized Paper-Based Microarray Chip for SERS Detection of Bacteria and Antibacterial and Photocatalytic Inactivation

作者：Afang Zhu, Shujat Ali, Zhen Wang, Yi Xu, Rongxi Lin, Tianhui Jiao, Qin Ouyang, Quansheng Chen · 发表于：Analytical Chemistry · 年份：2023 · DOI：10.1021/acs.analchem.3c03492 · 被引用次数：58 · 研究领域：Biosensors and Analytical Detection、Gold and Silver Nanoparticles Synthesis and Applications、Advanced Photocatalysis Techniques

Bacterial infections caused by pathogenic microorganisms have become a serious, widespread health concern. Thus, it is essential and required to develop a multifunctional platform that can rapidly and accurately determine bacteria and effectively inhibit or inactivate pathogens. Herein, a microarray SERS chip was successfully synthesized using novel metal/semiconductor composites (ZnO@Ag)-ZnO nanoflowers (ZnO NFs) decorated with Ag nanoparticles (Ag NPs) arrayed on a paper-based chip as a supporting substrate for in situ monitoring and photocatalytic inactivation of pathogenic bacteria. Typical Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli and Vibrio parahemolyticus were selected as models. Partial least-squares discriminant analysis (PLS-DA) was performed to minimize the dimensionality of SERS spectra data sets and to develop a cost-effective identification model. The classification accuracy was 100, 97.2, and 100% for S. aureus, E. coli, and V. parahemolyticus, respectively. The antimicrobial activity of ZnO@Ag was proved by the microbroth dilution method, and the minimum inhibitory concentrations (MICs) of S. aureus, E. coli, and V. parahemolyticus were 40, 50, and 55 μg/mL, respectively. Meanwhile, it demonstrated remarkable photocatalytic performance under natural sunlight for the inactivation of pathogenic bacteria, and the inactivation rates for S. aureus, E. coli, and V. parahemolyticus were 100, 97.03 and 97.56%, respectively. As a result, th...