Integrating genomic and multiomic data for Angelica sinensis provides insights into the evolution and biosynthesis of pharmaceutically bioactive compounds

作者：Shiming Li, Tsan‐Yu Chiu, Xin Jin, Dong Cao, Meng Xu, Mingzhi Zhu, Qi Zhou, Chun Liu, Yuan Zong, Shujie Wang, Kang Yu, Feng Zhang, Mingzhou Bai, Guangrui Liu, Yunlong Liang, Chi Zhang, Henrik Toft Simonsen, Jian Zhao, Baolong Liu, Shancen Zhao · 发表于：Communications Biology · 年份：2023 · DOI：10.1038/s42003-023-05569-5 · 被引用次数：25 · 研究领域：Metabolomics and Mass Spectrometry Studies、Traditional Chinese Medicine Analysis、Plant biochemistry and biosynthesis

Angelica sinensis roots (Angelica roots) are rich in many bioactive compounds, including phthalides, coumarins, lignans, and terpenoids. However, the molecular bases for their biosynthesis are still poorly understood. Here, an improved chromosome-scale genome for A. sinensis var. Qinggui1 is reported, with a size of 2.16 Gb, contig N50 of 4.96 Mb and scaffold N50 of 198.27 Mb, covering 99.8% of the estimated genome. Additionally, by integrating genome sequencing, metabolomic profiling, and transcriptome analysis of normally growing and early-flowering Angelica roots that exhibit dramatically different metabolite profiles, the pathways and critical metabolic genes for the biosynthesis of these major bioactive components in Angelica roots have been deciphered. Multiomic analyses have also revealed the evolution and regulation of key metabolic genes for the biosynthesis of pharmaceutically bioactive components; in particular, TPSs for terpenoid volatiles, ACCs for malonyl CoA, PKSs for phthalide, and PTs for coumarin biosynthesis were expanded in the A. sinensis genome. These findings provide new insights into the biosynthesis of pharmaceutically important compounds in Angelica roots for exploration of synthetic biology and genetic improvement of herbal quality.