The EMT-Related Genes GALNT3 and OAS1 are Associated with Immune Cell Infiltration and Poor Prognosis in Lung Adenocarcinoma

作者：Dan Luo, Mengying Fang, Shao Le, Jue Wang, Yuling Liang, Mengqin Chen, Xuemei Gui, Jie Yan, Wenjun Wang, Lili Yu, Xianming Fan, Qibiao Wu · 发表于：Frontiers in Bioscience-Landmark · 年份：2023 · DOI：10.31083/j.fbl2810271 · 被引用次数：10 · 研究领域：Ferroptosis and cancer prognosis、Cancer-related gene regulation、Cancer Immunotherapy and Biomarkers

Background: Lung cancer is the main cause of cancer-related death, with epithelial-mesenchymal transition (EMT) playing an important role in the development of this disease. The EMT-related genes Polypeptide N-Acetylgalactosaminyltransferase 3 (GALNT3) and 2′-5′-Oligoadenylate Synthetase 1 (OAS1) are involved in numerous tumor processes. Although these genes have been extensively studied in cancer, they have yet to be analyzed by multi-omics in lung adenocarcinoma (LUAD). Methods: EMT-related genes were identified by R and Venn diagram. Cox regression and Kaplan-Meier analysis were performed to evaluate patient survival, and the Gene Expression Profiling Interactive Analysis (GEPIA) database was used for correlation analysis. GeneCards and R packages were used to explore gene characterization and functional annotation. The Tumor Immune Estimation Resource (TIMER), Human Protein Atlas (HPA), University of Alabama at Birmingham Cancer (UALCAN), and The Cancer Genome Atlas (TCGA) databases were used to investigate gene expression, which was then confirmed by RT-PCR. Clinicopathological analysis was carried out using the UALCAN database. Functional mechanisms and multi-omics analysis were performed using DNA Methylation Interactive Visualization Database (DNMIVD), Targetscan, TIMER, Tumor–immune System Interactions Database (TISIDB) and cBioportal. Diagnostic values were calculated using ROC curve analysis. Results: A total of 320 EMT-related genes were identified in LUAD. Their ...