Sources of gut microbiota variation in a large longitudinal Finnish infant cohort

作者：R. Jokela, Alise J. Ponsero, Evgenia Dikareva, Xiaodong Wei, Kaija‐Leena Kolho, Katri Korpela, Willem M. de Vos, Anne Salonen · 发表于：EBioMedicine · 年份：2023 · DOI：10.1016/j.ebiom.2023.104695 · 被引用次数：48 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Oral microbiology and periodontitis research

BACKGROUND: Although the infant gut microbiota has been extensively studied, comprehensive assessment on the microbiota determinants including technical variables has not been performed in large infant cohorts. METHODS: We studied the effect of 109 variables on the 16S rRNA gene amplicon-based gut microbiota profiles of infants sampled longitudinally from three weeks to two years of life in the Finnish HELMi birth cohort. Spot faecal samples from both parents were included for intra-family analyses, totalling to 7657 samples from 985 families that were evaluated for beta-diversity patterns using permutational multivariate analysis on Bray-Curtis distances, and differential abundance testing and alpha-diversity for variables of interest. We also assessed the effect of different taxonomic levels and distance methods. FINDINGS: In time point-specific models, the largest share of variation explained, up to 2-6%, were seen in decreasing order for the DNA extraction batch, delivery mode and related perinatal exposures, defecation frequency and parity/siblings. Variables describing the infant gastrointestinal function were continuously important during the first two years, reflecting changes in e.g., feeding habits. The effect of parity/siblings on infant microbiota was modified by birth mode and exposure to intrapartum antibiotics, exemplifying the tight interlinkage of perinatal factors relevant for infant microbiota research. In total, up to 19% of the biological microbiota varia...