In vitro fermentation of epilactose and epilactitol by human faecal microbiota

作者：Yu-Wei Zhang, Yu Qiao, Xiaoqing Xu, Qing Peng, Jinwei Ren, Lan Ma, Dandan Tian, Yuxin Gong, Deqin Feng, Bo Shi · 发表于：International Dairy Journal · 年份：2023 · DOI：10.1016/j.idairyj.2023.105697 · 被引用次数：11 · 研究领域：Probiotics and Fermented Foods、Gut microbiota and health、Digestive system and related health

To obtain new lactose-derived prebiotic , epilactose and its derivative epilactitol were synthesised with high purity (>98%), and their structural characteristics were investigated. To determine the prebiotic potential of epilactose and epilactitol, these two compounds were fermented in vitro with human faecal microbiota . Consequently, epilactose addition increased the relative abundance of Bifidobacterium , Megamonas , Blautia , and Phascolarctobacterium , whereas epilactitol addition enhanced the relative abundance of Anaerostipes , Megamonas , Blautia and Phascolarctobacterium after in vitro fermentation for 24 h. The two compounds increased the production of total amount of short-chain fatty acids (SCFAs); in particular, epilactose addition increased acetate and n -butyrate production ( P < 0.05), and epilactitol addition increased propionate and n -butyrate production ( P < 0.05) after in vitro fermentation for 24 h. In conclusion, epilactose and epilactitol can be utilised by human faecal microbiota , modify microbial community , increase SCFA production , they may serve as novel prebiotics to bring health benefits for humans.