Expression profile of cytokines and chemokines in a mouse high-altitude cerebral edema model

作者：Zibi Shi, Xiufang Jiang, Yanan Geng, Xiangpei Yue, Jiayue Gao, Xiang Cheng, Ming Zhao, Lingling Zhu · 发表于：International Journal of Immunopathology and Pharmacology · 年份：2023 · DOI：10.1177/03946320231177189 · 被引用次数：17 · 研究领域：High Altitude and Hypoxia、Cardiovascular and Diving-Related Complications、Neuroscience of respiration and sleep

Introduction High-altitude cerebral edema (HACE) is considered to be the end-stage of acute mountain sickness (AMS); however, its pathophysiological mechanism remains unknown. Increasing evidences support that inflammation is an important risk factor for the occurrence of HACE. Including our published papers, previous studies demonstrated that the levels of IL-6, IL-1β, and TNF-α in both serum and hippocampus were increased in the mouse HACE model induced by LPS stimulation combined with hypobaric hypoxia exposure; however, the expression profile of other cytokines and chemokines remains unknown. Objective This study was to analyze the expression profile of cytokines and chemokines in the HACE model. Methods The mouse HACE model was established by LPS stimulation combined with hypobaric hypoxia exposure (LH). The mice were divided into the normoxic group, LH-6 h group, LH-1 d group, and LH-7 d group. Brain water content (BWC) was determined using the wet/dry weight ratio. The levels of 30 cytokines and chemokines in the serum and hippocampal tissue were detected using LiquiChip. The mRNA expression of cytokines and chemokines in hippocampal tissue were determined by q-PCR. Results In the current study, we found that the brain water content was increased after the combinational treatment of LPS and hypobaric hypoxia. The results of LiquiChip showed that, in the serum and hippocampal tissue, most factors in all 30 cytokines and chemokines were dramatically upregulated at 6 h, a...