Intermittent fasting modulates the intestinal microbiota and improves obesity and host energy metabolism

作者：Xiangwei Hu, Kai Xia, Minhui Dai, Xiao-Feng Han, Peng Yuan, Jia Liu, Shiwei Liu, Fuhuai Jia, Jiayu Chen, Fangfang Jiang, Jieyao Yu, Huanming Yang, Jian Wang, Xun Xu, Xin Jin, Karsten Kristiansen, Liang Xiao, Wei Chen, Mo Han, Shenglin Duan · 发表于：npj Biofilms and Microbiomes · 年份：2023 · DOI：10.1038/s41522-023-00386-4 · 被引用次数：60 · 研究领域：Dietary Effects on Health、Gut microbiota and health、Diet and metabolism studies

Intermittent fasting (IF) is a promising paradigm for weight loss which has been shown to modulate the gut microbiota based on 16S rRNA gene amplicon sequencing. Here, 72 Chinese volunteers with a wide range of body mass index (BMI) participated in a three-week IF program during which an average loss of 3.67 kg body weight accompanied with improved clinical parameters was observed irrespective of initial anthropometric and gut microbiota status. Fecal samples were collected before and after the intervention and subjected to shotgun metagenomic sequencing. De novo assembly yielded 2934 metagenome-assembled genomes (MAGs). Profiling revealed significant enrichment of Parabacteroides distasonis and Bacteroides thetaiotaomicron after the intervention, with inverse correlations between their relative abundances and parameters related to obesity and atherosclerotic cardiovascular diseases (ASCVD). MAGs enriched after the intervention showed high richness and diversity of carbohydrate-active enzymes, with an increased relative abundances of genes related to succinate production and glutamate fermentation.