Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage

作者：Zhihui Fu, Lin Sun, Meiling Hou, Junfeng Hao, Qiang Lü, Tingyu Liu, Xiuzhen Ren, Yushan Jia, Zhijun Wang, Gentu Ge · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.971449 · 被引用次数：38 · 研究领域：Ruminant Nutrition and Digestive Physiology、Probiotics and Fermented Foods、Gut microbiota and health

In this study, we analyzed the fermentation quality, microbial community, and metabolome characteristics of ryegrass silage from different harvests (first harvest-AK, second harvest-BK, and third harvest-CK) and analyzed the correlation between fermentative bacteria and metabolites. The bacterial community and metabolomic characteristics were analyzed by single-molecule real-time (SMRT) sequencing and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS), respectively. After 60 days of ensiling, the pH of BK was significantly lower than those of AK and CK, and its lactic acid content was significantly higher than those of AK and CK. Lactiplantibacillus and Enterococcus genera dominate the microbiota of silage obtained from ryegrass harvested at three different harvests. In addition, the BK group had the highest abundance of Lactiplantibacillus plantarum (58.66%), and the CK group had the highest abundance of Enterococcus faecalis (42.88%). The most annotated metabolites among the differential metabolites of different harvests were peptides, and eight amino acids were dominant in the composition of the identified peptides. In the ryegrass silage, arginine, alanine, aspartate, and glutamate biosynthesis had the highest enrichment ratio in the metabolic pathway of KEGG pathway enrichment analysis. Valyl-isoleucine and glutamylvaline were positively correlated with Lactiplantibacillus plantarum . D-Pipecolic acid and L-glutamic acid were positively correlat...