High Throughput Screening for Bioactive Components of Berberis baluchistanica Ahrendt Root and Their Functional Potential Assessment

作者：Zareen Gul, Ali Akbar, Imran Ali, Javed Muhammad, Zia Ur Rehman, Amna Bano, Abdul Samad, Amjad Khan, Sami Ullah, Saadullah Khan Leghari, Su Hlaing Chein, Ali A. Rabaan · 发表于：BioMed Research International · 年份：2022 · DOI：10.1155/2022/1746116 · 被引用次数：8 · 研究领域：Berberine and alkaloids research、Ethnobotanical and Medicinal Plants Studies、Psidium guajava Extracts and Applications

Berberis baluchistanica Ahrendt is a medicinal plant potentially known for the treatment of different diseases. The bioactive, antioxidant, nutritional components, and antimicrobial properties of crude ethanolic root extract of Berberis baluchistanica were evaluated in this study. The extract was analyzed for total phenolic, flavonoid, DPPH (2, 2‐diphenyl‐1‐picryl‐hydrazyl) scavenging ability, FRAP (ferric reducing antioxidant power), nutritional, and antimicrobial potentials. The alkaloids, tannins, cardiac glycosides, anthraquinones, coumarin, saponins, phenolics, flavonoids, steroids, and terpenoids were confirmed. The extract possessed DPPH radical inhibition with the IC 50 of 1.125 mg/mL and FRAP % reduction activity with IC 50 (0.912 mg/mL). Total phenolic 19.897 ± 4.8141 mg GAE/g and flavonoid 12.9876 ± 0.8388 mg QE/g contents were confirmed in the root. The extracts exhibit good antibacterial activity against a broad spectrum of food borne pathogens Pseudomonas aeruginosa , Salmonella typhi , Klebsiella pneumoniae , Escherichia coli , and Staphylococcus aureus . The highest inhibitory activity was against Escherichia coli 23.30 ± 1.16 mm and lowest against Klebsiella pneumoniae 7 ± 0.01 mm. Furthermore, the presence of various phytochemical constituents (plant secondary metabolites) was also confirmed with gas chromatography and mass spectroscopy analysis. Results disclosed the occurrence of more than 70 compounds possessing various medicinal properties supporting the...