Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation

作者：Qingbin Guo, Zhengxin Shan, Yanhui Shao, Nifei Wang, Keying Qian, H. Douglas Goff, Qi Wang, Steve W. Cui, Huihuang Ding · 发表于：Polymers · 年份：2022 · DOI：10.3390/polym14132667 · 被引用次数：12 · 研究领域：Polysaccharides and Plant Cell Walls、Seaweed-derived Bioactive Compounds、Microbial Metabolites in Food Biotechnology

The pectic polysaccharides extracted from flaxseed (Linum usitatissiumum L.) mucilage and kernel were characterized as rhamnogalacturonan-I (RG-I). In this study, the conformational characteristics of RG-I fractions from flaxseed mucilage and kernel were investigated, using a Brookhaven multi-angle light scattering instrument (batch mode) and a high-performance size exclusion chromatography (HPSEC) system coupled with Viscotek tetra-detectors (flow mode). The Mw of flaxseed mucilage RG-I (FM-R) was 285 kDa, and the structure-sensitive parameter (ρ) value of FM-R was calculated as 1.3, suggesting that the FM-R molecule had a star-like conformation. The Mw of flaxseed kernel RG-I (FK-R) was 550 kDa, and the structure-sensitive parameter (ρ) values ranged from 0.90 to 1.21, suggesting a sphere to star-like conformation with relatively higher segment density. The correlation between the primary structure and conformation of RG-I was further discussed to better understand the structure–function relationship, which helps the scale-up applications of pectins in food, pharmaceutical, or cosmetic industries.