Elucidating Therapeutic and Biological Potential of Berberis baluchistanica Ahrendt Bark, Leaf, and Root Extracts

作者：Zareen Gul, Ali Akbar, Saadullah Khan Leghari · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.823673 · 被引用次数：21 · 研究领域：Essential Oils and Antimicrobial Activity、Ethnobotanical and Medicinal Plants Studies、Berberine and alkaloids research

Berberis baluchistanica Ahrendt is a medicinal plant known to have potential for the treatment of various diseases. In the present study, the ethanolic extracts of the bark, leaves, and roots of B. baluchistanica plant were evaluated for in vitro antimicrobial, anti-leishmanial, anticancer, and anti-inflammatory activities. The antibacterial and antifungal activities were determined by agar mix and agar well diffusion method. All extracts showed potential activity against the target bacteria ( Bacillus subtilis , Bacillus licheniformis , Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Rhodococcus erythropolis , Salmonella typhi , and Staphylococcus aureus ) and fungal strains ( Aspergillus flavus , Aspergillus niger , and Mucor mucedo ). S. aureus proved to be the most sensitive strain for each extract, with a maximum zone of inhibition for bark at 23 ± 0.12 mm, for leaves at 22 ± 0.36 mm, and for root extracts at 20.21 ± 0.06 mm). The minimum inhibitory concentration values of B. baluchistanica bark, leaves, and roots for different target bacterial strains ranged from 1.56 to 25 mg ml –1 , and the minimum bactericidal concentrations were in the range of 3.12 to 25 mg ml –1 , respectively. The root extract possessed potent antifungal activity against A. flavus with 83% of growth inhibition, A. niger with 80%, and M. mucedo with 73%. The bark extract was found active against M. mucedo with 86% of inhibition, followed by 70% against A. flavus and 60% against...