Reclassification of Olsenella gallinarum as Thermophilibacter gallinarum comb. nov. and description of Thermophilibacter immobilis sp. nov., isolated from the mud in a fermentation cellar used for the production of Chinese Luzhou-flavour Baijiu

作者：Lingfei Lu, Yang Yang, Lei Zheng, Rui Zhang, Guang-Qian Liu, Tingyao Tu, Tao Xu, Xue Luo, Maofang Ran, Liqiang Zhang, Songtao Wang, Caihong Shen, Yinggang Zhang · 发表于：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 年份：2021 · DOI：10.1099/ijsem.0.005192 · 被引用次数：20 · 研究领域：Genomics and Phylogenetic Studies、Microbial Community Ecology and Physiology、Probiotics and Fermented Foods

A novel Gram-stain-positive, strictly anaerobic, elliptical, non-motile and non-flagellated bacterium, designed LZLJ-2 T , was isolated from the mud in a fermentation cellar used for the production of Chinese Luzhou-flavour Baijiu. Growth occurred at 28–45 °C (optimum, 37 °C), at pH 6.0–7.0 (optimum, pH 6.0) and with concentrations of NaCl up to 2 % (w/v; optimum, 0 %). On the basis of 16S rRNA gene sequence similarity, strain LZLJ-2 T belonged to the genus Thermophilibacter and was most closely related to Thermophilibacter mediterraneus Marseille-P3256 T (similarity 96.9 %), Olsenella gallinarum ClaCZ62 T (similarity 96.6 %) and Thermophilibacter provencensis Marseille-P2912 T (similarity 96.4 %). In addition, strain LZLJ-2 T had high similarity to the genus Olsenella , including Olsenella profusa DSM 13989 T (similarity 94.9 %), Olsenella umbonata DSM 22620 T (similarity 94.9 %), Olsenella uli ATCC 49627 T (similarity 94.22 %), Tractidigestivibacter scatoligenes DSM 28304 T (similarity 93.9 %) and Paratractidigestivibacter faecalis KCTC 15699 T (similarity 93.25 %). Comparative genome analysis showed that orthoANI values between strain LZLJ-2 T and Thermophilibacter mediterraneus Marseille-P3256 T , Olsenella gallinarum ClaCZ62 T , Thermophilibacter provencensis Marseille-P2912 T , Olsenella profusa DSM 13989 T , Olsenella umbonata DSM 22620 T , Olsenella uli ATCC 49627 T , Tractidigestivibacter scatoligenes DSM 28304 T and Paratractidigestivibacter faecalis KCTC 15699 T we...