Effect of epiphytic microbiota from napiergrass and Sudan grass on fermentation characteristics and bacterial community in oat silage

作者：Siran Wang, Junfeng Li, Jie Zhao, Zhihao Dong, Dong Dong, Tao Shao · 发表于：Journal of Applied Microbiology · 年份：2021 · DOI：10.1111/jam.15293 · 被引用次数：18 · 研究领域：Ruminant Nutrition and Digestive Physiology、Probiotics and Fermented Foods、Gut microbiota and health

AIMS: To investigate the effects of epiphytic microbiota from napiergrass and Sudan grass on ensiling characteristics and microbial community of oat silage. METHODS AND RESULTS: By γ-ray irradiation sterilization and microbiota transplantation technology, the sterilized oat was inoculated as follows: (a) aseptic water (STOT), (b) epiphytic bacteria on oat (OTOT), (c) epiphytic bacteria on napiergrass (OTNP) and (d) epiphytic bacteria on Sudan grass (OTSD). STOT remained in the unfermented state based on similar chemical components with fresh oat. Compared with OTOT and OTSD, higher lactic acid content and ratio of lactic acid to acetic acid, and lower pH, acetic acid and ammonia nitrogen contents were observed in OTNP after 60 days of ensiling. At the late stage, Lactobacillus was the most predominant in each group. Lactococcus was eventually replaced by Lactobacillus in OTSD, whereas Lactococcus was found throughout the whole ensiling process in OTNP. Higher abundance of Weissella was observed in OTSD at the early and late stages. The result of co-occurrence network analysis proved that Lactococcus was pivotal in determining the silage fermentation pattern during ensiling. According to the 16S rRNA gene-predicted functional profiles, the inoculation of epiphytic microbiota from oat enhanced the metabolism of amino acids, whereas the inoculation of epiphytic microbiota from napiergrass and Sudan grass accelerated the carbohydrate metabolism. CONCLUSIONS: The epiphytic microbi...