Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health

作者：Thi Phuong Nam Bui, Louise Mannerås-Holm, Robert Puschmann, Hao Wu, Antonio Dario Troise, Bart Nijsse, Sjef Boeren, Fredrik Bäckhed, Dorothea Fiedler, Willem M. de Vos · 发表于：Nature Communications · 年份：2021 · DOI：10.1038/s41467-021-25081-w · 被引用次数：238 · 研究领域：Phytase and its Applications、Folate and B Vitamins Research、Muscle metabolism and nutrition

Abstract We describe the anaerobic conversion of inositol stereoisomers to propionate and acetate by the abundant intestinal genus Anaerostipes . A inositol pathway was elucidated by nuclear magnetic resonance using [ 13 C]-inositols, mass spectrometry and proteogenomic analyses in A. rhamnosivorans , identifying 3-oxoacid CoA transferase as a key enzyme involved in both 3-oxopropionyl-CoA and propionate formation. This pathway also allowed conversion of phytate-derived inositol into propionate as shown with [ 13 C]-phytate in fecal samples amended with A. rhamnosivorans . Metabolic and (meta)genomic analyses explained the adaptation of Anaerostipes spp. to inositol-containing substrates and identified a propionate-production gene cluster to be inversely associated with metabolic biomarkers in (pre)diabetes cohorts. Co-administration of myo-inositol with live A. rhamnosivorans in western-diet fed mice reduced fasting-glucose levels comparing to heat-killed A. rhamnosivorans after 6-weeks treatment. Altogether, these data suggest a potential beneficial role for intestinal Anaerostipes spp. in promoting host health.