Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism

作者：Gui-Li Huang, Lingxiang Sun, Jiajia Ma, Si-Yao Sui, Yuning Wang · 发表于：Bioengineered · 年份：2021 · DOI：10.1080/21655979.2021.1886387 · 被引用次数：9 · 研究领域：Phytochemicals and Antioxidant Activities、Garlic and Onion Studies、Plant biochemistry and biosynthesis

This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC50 value of 21.03 ± 2.37 μg/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry.