Functional Analysis of Sugars in Modulating Bacterial Communities and Metabolomics Profiles of Medicago sativa Silage

作者：Bing Wang, Run Gao, Zhe Wu, Zhu Yu · 发表于：Frontiers in Microbiology · 年份：2020 · DOI：10.3389/fmicb.2020.00641 · 被引用次数：31 · 研究领域：Ruminant Nutrition and Digestive Physiology、Probiotics and Fermented Foods、Phytoestrogen effects and research

This study explored the effects of four sugar source treatments, including no sugar (CON), fructose (FRU), pectin (PEC), and starch (STA), on the microbiota and metabolome of alfalfa (Medicago sativa) silage. The bacterial community was determined via 16S rRNA gene sequencing. The metabolome was analyzed using ultra high-performance liquid chromatography tandem time-of-flight mass spectrometry. After 60 d of fermentation, the pH values in FRU and PEC were lower than those in STA and CON. FRU had a greater lactic acid concentration compared to STA and CON. Weissella (47.44%) and Lactobacillus (42.13%) were the dominant species in all four groups. The abundance of Pediococcus was lower and the abundance of Leuconostoc, Pantoea, and Microbacterium was higher in FRU compared to CON. The abundance of norank_f__Bacteroidales_S24-7_group was higher and the abundance of Turicibacter was lower in both FRU and PEC than in CON. Leuconostoc was negatively correlated with the pH value and Pediococcus was positively correlated with the pH value. No microbiomes were detected as discriminative features between STA and CON. The addition of FRU and PEC presented more peptides, such as Leu-Val-Thr, Leu-Phe, Ile-Pro-Ile, Val-Trp, and Ile-Leu-Leu but a lower abundance of metabolites for triterpene glycosides including sanchinoside B1, medicagenic acid, betavulgaroside IV, and prosapogenin compared to CON. The addition of PEC presented more phenyllactic acid compared to CON. Our study demonstrated...