A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans

作者：Haiqiang Chen, Shuang Wang, Aimei Zhou, Jianyin Miao, Jingyi Liu, Soottawat Benjakul · 发表于：Journal of Functional Foods · 年份：2020 · DOI：10.1016/j.jff.2020.103907 · 被引用次数：52 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、Protein Hydrolysis and Bioactive Peptides、Insect Utilization and Effects

The residue rich in protein (up to 88.78%) obtained from round scad (Decapterus maruadsi) after oil extraction, was hydrolyzed by Alcalase to produce antioxidative peptides. The hydrolysate was separated by ultrafiltration, Sephadex G-15 and RP-HPLC, and the active fractions were screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline- 6 sulphonic acid) diammonium salt (ABTS), reducing power and oxygen radical absorbance capacity (ORAC). The obtained peptide with the highest antioxidative activity was then analyzed by HPLC and LC-MS/MS and its anti-aging effect was examined using Caenorhabditis elegans (C. elegans) model. The peptide (Ile-Leu-Gly-Ala-Thr-Ile-Asp-Asn-Ser-Lys, 1153.4 Da), identified by LC-MS/MS showed the highest antioxidant activity. C. elegans fed with the selected peptide had longer lifespan, higher survival rate, superoxide dismutase (SOD) and catalase (CAT) activities than the control group (P＜0.05), demonstrating the promising anti-aging effects of the antioxidant peptide from defatted round scad.