Overexpression of Mps1 in colon cancer cells attenuates the spindle assembly checkpoint and increases aneuploidy

作者：Youguo Ling, Xiaojuan Zhang, Yuanyuan Bai, Ping Li, Congwen Wei, Ting Song, Zirui Zheng, Kai Guan, Yanhong Zhang, Buchang Zhang, Xuedong Liu, Z. Runlin, Cheng Cao, Hui Zhong, Quanbin Xu · 发表于：Biochemical and Biophysical Research Communications · 年份：2014 · DOI：10.1016/j.bbrc.2014.07.071 · 被引用次数：52 · 研究领域：Microtubule and mitosis dynamics、Ubiquitin and proteasome pathways、Cancer-related Molecular Pathways

The spindle assembly checkpoint kinase Mps1 is highly expressed in several types of cancers, but its cellular involvement in tumorigenesis is less defined. Herein, we confirm that Mps1 is overexpressed in colon cancer tissues. Further, we find that forced expression of Mps1 in the colon cancer cell line SW480 enables cells to become resistant to both Mps1 inhibition-induced checkpoint depletion and cell death. Overexpression of Mps1 also increases genome instability in tumor cells owing to a weakened spindle assembly checkpoint. Collectively, our findings suggest that high levels of Mps1 contribute to tumorigenesis by attenuating the spindle assembly checkpoint.