Comparative Genomic and Functional Analysis of Lactobacillus casei and Lactobacillus rhamnosus Strains Marketed as Probiotics

作者：Franï¿ ⁄ ois P. Douillard, Angela Ribbera, Hanna M. Jï¿ ⁄ rvinen, Ravi Kant, Taija E. Pietilï¿ ⁄, Cinzia Lucia Randazzo, Lars Paulín, Pia Laine, Cinzia Caggia, Ingemar von Ossowski, Justus Reunanen, Reetta Satokari, Seppo Salminen, Airi Palva, Willem M. de Vos · 发表于：Applied and Environmental Microbiology · 年份：2013 · DOI：10.1128/aem.03467-12 · 被引用次数：126 · 研究领域：Probiotics and Fermented Foods、Microbial Metabolites in Food Biotechnology、Gut microbiota and health

Four Lactobacillus strains were isolated from marketed probiotic products, including L. rhamnosus strains from Vifit (Friesland Campina) and Idoform (Ferrosan) and L. casei strains from Actimel (Danone) and Yakult (Yakult Honsa Co.). Their genomes and phenotypes were characterized and compared in detail with L. casei strain BL23 and L. rhamnosus strain GG. Phenotypic analysis of the new isolates indicated differences in carbohydrate utilization between L. casei and L. rhamnosus strains, which could be linked to their genotypes. The two isolated L. rhamnosus strains had genomes that were virtually identical to that of L. rhamnosus GG, testifying to their genomic stability and integrity in food products. The L. casei strains showed much greater genomic heterogeneity. Remarkably, all strains contained an intact spaCBA pilus gene cluster. However, only the L. rhamnosus strains produced mucus-binding SpaCBA pili under the conditions tested. Transcription initiation mapping demonstrated that the insertion of an iso-IS30 element upstream of the pilus gene cluster in L. rhamnosus strains but absent in L. casei strains had constituted a functional promoter driving pilus gene expression. All L. rhamnosus strains triggered an NF-κB response via Toll-like receptor 2 (TLR2) in a reporter cell line, whereas the L. casei strains did not or did so to a much lesser extent. This study demonstrates that the two L. rhamnosus strains isolated from probiotic products are virtually identical to L. ...